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Read Moreused to examiiic plant inputs to the food webs, trophic organization, and the efficiency of carbon transfer through ecosystems. 13 12 In several respects, the biomass C/ C ratio is an especially suitable parameter for such a study. Organic carbon is the major con stituent of biomass, and can easily be extracted and analyzed. The 1
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Read Morepyridoxal-5-phosphate (PLP) or both PLP and inhibitor aminoethoxyvinylglycine have been determined by x-ray crystallography. The structures showed good con-servation of the catalytic residues, suggesting a similar catalytic mechanism for ACS and other PLP-dependent enzymes. However, the proximity of Tyr152 to the C- –S
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Read MorePLP (Fig. la). The major actin-binding contact defined in the vertebrate actin-profilin crystal consists of hydrophobic and basic amino acids positioned on the adjacent face, pri- marily on ~-strands 4, 5 and 6 and the C-terminal a- helix 1~,2~ (see Fig. lb). This spatial separation of PLP- and
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Read MoreIsolates used in this study. All isolates of Embellisia and Nimbya were obtained from the collection of E. G. Simmons (TABLE I). Other isolates in this work were obtained from various sources, and many of the isolates have been includ-ed in previous phylogenetic work (Pryor and Gilbertson 2000; TABLE I). All isolates were cultured on 0.053 PDA
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Read MorePLP ATP (4) Pi (3) I II I A Interconversion of B 6-vitamers I Reactions of the salvage pathway (1), (2) II Reactions before (3) and after (4) passage through a cell membrane (e.g. blood brain barrier) B Metabolism of neurotransmitter glutamate γ −aminobutyric acid (GABA) PLP CO 2 (5) Fig. 2. Reactions potentially affected by B
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Read Moreexposed for 4 h along with a solution of pure PLP as a con- ... a naturally occurring plant. ... can lead to an insufficient supply of available PLP (e.g. pyridox(am)ine 5'-phosphate oxidase ...
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Read Morewas resuspended in 2.5 ml of a solution containing 100 mmol Na-phosphate (pH 7.8), 5 lM PLP, 0.5 mmol 2-ME, and 10% glycerol. The preparations of the enzymes werecarriedoutat4 C(Satohandothers1997).ACSactivity was assayed by incubating a 100-ll enzyme solution (1.29 mg protein) with 100 mmol 2-[4-(2-hydroxyethyl)-1-
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Read More(PM), pyridoxine 5'-phosphate (PNP), pyridoxal 5'-phosphate (PLP), and pyridoxamine 5'-phosphate (PMP) [6, 7]. PNPO converts PNP and PMP to PLP, the biologically active form of VB6, which is a co-factor for more than 140 different enzymes that are involved not only in the metabolism of amino acids,
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Add: No. 1688, Gaoke East Road, Pudong new district, Shanghai, China.
